Protein_Domain
SgsE
Part:BBa_K525301:Design
Designed by: Timo Wolf Group: iGEM11_Bielefeld-Germany (2011-09-10)
S-Layer SgsE from Geobacillus stearothermophilus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 95
Illegal BglII site found at 950 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 2329 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1585
Design Notes
Truncated sgsE gene from Geobacillus stearothermophilus NRS 2004/3a missing the first 330 amino acids
- it was shown that these first 330 amino acid are not necessary for self-assembly
Source
- Organism: Geobacillus stearothermophilus NRS 2004/3a
- synthesized, codon-optimized for Escherichia coli
References
Schäffer C, Novotny R, Küpcü R, Zayni S, Scheberl A, Friedmann J, Sleytr UB, Messner P (2007) Novel Biocatalysts Based on S-Layer Self-Assembly of Geobacillus Stearothermophilus NRS 2004/3a: A Nanobiotechnological Approach, [http://onlinelibrary.wiley.com/doi/10.1002/smll.200700200/pdf Small 3(9):1549-1559].