Protein_Domain
SgsE

Part:BBa_K525301:Design

Designed by: Timo Wolf   Group: iGEM11_Bielefeld-Germany   (2011-09-10)

S-Layer SgsE from Geobacillus stearothermophilus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
    Illegal BglII site found at 950
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 2329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1585


Design Notes

Truncated sgsE gene from Geobacillus stearothermophilus NRS 2004/3a missing the first 330 amino acids

  • it was shown that these first 330 amino acid are not necessary for self-assembly


Source

  • Organism: Geobacillus stearothermophilus NRS 2004/3a
  • synthesized, codon-optimized for Escherichia coli


References

Schäffer C, Novotny R, Küpcü R, Zayni S, Scheberl A, Friedmann J, Sleytr UB, Messner P (2007) Novel Biocatalysts Based on S-Layer Self-Assembly of Geobacillus Stearothermophilus NRS 2004/3a: A Nanobiotechnological Approach, [http://onlinelibrary.wiley.com/doi/10.1002/smll.200700200/pdf Small 3(9):1549-1559].